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The response of the marine bacterium Sphingopyxis alaskensis to solar radiation assessed by quantitative proteomics

Identifieur interne : 007D49 ( Main/Exploration ); précédent : 007D48; suivant : 007D50

The response of the marine bacterium Sphingopyxis alaskensis to solar radiation assessed by quantitative proteomics

Auteurs : S. Matallana-Surget [France] ; F. Joux [France] ; M. J. Raftery [Australie] ; R. Cavicchioli [Australie]

Source :

RBID : ISTEX:B9D93EEBDAD46D694822C16DFF371EABA9CEC2FC

Descripteurs français

English descriptors

Abstract

The adaptive response of the marine bacterium Sphingopyxis alaskensis RB2256 to solar radiation (both visible and ultraviolet) was assessed by a quantitative proteomic approach using iTRAQ (isobaric tags for relative and absolute quantification). Both growth phase (mid‐log and stationary phase) and duration (80 min or 8 h) of different light treatments (combinations of visible light, UV‐A and UV‐B) were assessed relative to cultures maintained in the dark. Rates of total protein synthesis and viability were also assessed. Integrating knowledge from the physiological experiments with quantitative proteomics of the 12 conditions tested provided unique insight into the adaptation biology of UV and visible light responses of S. alaskensis. High confidence identifications were obtained for 811 proteins (27% of the genome), 119 of which displayed significant quantitative differences. Mid‐log‐phase cultures produced twice as many proteomic changes as stationary‐phase cultures, while extending the duration of irradiation exposure of stationary‐phase cultures did not increase the total number of quantitative changes. Proteins with significant quantitative differences were identified that were characteristic of growth phase and light treatment, and cellular processes, pathways and interaction networks were determined. Key factors of the solar radiation adaptive response included DNA‐binding proteins implicated in reducing DNA damage, detoxification of toxic compounds such as glyoxal and reactive oxygen species, iron sequestration to minimize oxidative stress, chaperones to control protein re/folding, alterations to nitrogen metabolism, and specific changes to transcriptional and translational processes.

Url:
DOI: 10.1111/j.1462-2920.2009.01992.x


Affiliations:


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<term>Abundance</term>
<term>Abundance ratio</term>
<term>Acid aminotransferase</term>
<term>Adaptive</term>
<term>Adaptive response</term>
<term>Alaskensis</term>
<term>Antitermination protein nusg</term>
<term>Appl</term>
<term>Appl environ microbiol</term>
<term>Bacterium</term>
<term>Beta subunit</term>
<term>Biol chem</term>
<term>Biological replicates</term>
<term>Biosystems</term>
<term>Blackwell publishing</term>
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<term>Chaperonin protein</term>
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<term>Charge state</term>
<term>Climate change</term>
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<term>Colour code</term>
<term>Common proteins</term>
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<term>Dantas caldas</term>
<term>Dark control</term>
<term>Different growth conditions</term>
<term>Different light treatments</term>
<term>Differential abundance</term>
<term>Differential protein abundance</term>
<term>Elongation factor</term>
<term>Environ</term>
<term>Environmental microbiology</term>
<term>Escherichia coli</term>
<term>Family protein</term>
<term>Fegatella</term>
<term>Formic acid</term>
<term>Functional relationships</term>
<term>Gene expression</term>
<term>Genome sequence</term>
<term>Glutamine synthetase</term>
<term>Glyoxalase</term>
<term>Growth conditions</term>
<term>Growth phase</term>
<term>Heat shock protein</term>
<term>Hierarchical cluster analysis</term>
<term>Higher abundance</term>
<term>Host factor subunit</term>
<term>Hydrogen peroxide</term>
<term>Hydrogen peroxide resistance</term>
<term>Interaction networks</term>
<term>Iron homeostasis</term>
<term>Iron storage</term>
<term>Irradiation conditions</term>
<term>Irradiation treatment</term>
<term>Irradiation treatments</term>
<term>Isobaric tags</term>
<term>Itraq</term>
<term>Itraq labelling</term>
<term>Itraq reagents</term>
<term>Joux</term>
<term>Light conditions</term>
<term>Light exposure</term>
<term>Light treatment</term>
<term>Light treatments</term>
<term>Liquid chromatography tandem mass spectrometry</term>
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<term>Lower abundance</term>
<term>Manual annotation</term>
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<term>Marine bacterium</term>
<term>Marine bacterium sphingopyxis alaskensis</term>
<term>Marine ecosystems</term>
<term>Marine environment</term>
<term>Marine oligotrophic ultramicrobacterium</term>
<term>Membrane lipoprotein</term>
<term>Metabolic pathways</term>
<term>Metabolism</term>
<term>Methanococcoides burtonii</term>
<term>Michrom bioresources</term>
<term>Microb ecol</term>
<term>Microbiol</term>
<term>Microbiology</term>
<term>Ml8h</term>
<term>Ml8h cells</term>
<term>Ml8h treatment</term>
<term>Molecular mechanisms</term>
<term>Nitrogen metabolism</term>
<term>Nucleic acids</term>
<term>Nutrient limitation</term>
<term>Ocean waters</term>
<term>Oligotrophic</term>
<term>Ompa</term>
<term>Ompa family</term>
<term>Ostrowski</term>
<term>Outer membrane protein ompa</term>
<term>Oxidative</term>
<term>Oxidative damage</term>
<term>Oxidative stress</term>
<term>Oxidative stress response</term>
<term>Pathway</term>
<term>Peptide</term>
<term>Periplasmic family protein</term>
<term>Perkin elmer</term>
<term>Pfkb family carbohydrate kinase_putative adenosine kinase</term>
<term>Photochem photobiol</term>
<term>Polymerase subunit omega</term>
<term>Positive gravy indices</term>
<term>Precursor</term>
<term>Previous studies</term>
<term>Proc natl acad</term>
<term>Protein</term>
<term>Protein abundance</term>
<term>Protein abundance changes</term>
<term>Protein dnak</term>
<term>Protein extraction</term>
<term>Protein family</term>
<term>Protein samples</term>
<term>Protein synthesis</term>
<term>Proteome</term>
<term>Proteomic</term>
<term>Proteomic analysis</term>
<term>Proteomic changes</term>
<term>Proteomic response</term>
<term>Proteomics</term>
<term>Proteomics data sets</term>
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<term>Quantitative proteomics</term>
<term>Quantitative proteomics data</term>
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<term>Ratio ratio</term>
<term>Ratio values</term>
<term>Reactive oxygen species</term>
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<term>Relative protein rate</term>
<term>Ribosomal</term>
<term>Ribosomal protein</term>
<term>Ribosomal proteins</term>
<term>Room temperature</term>
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<term>Sequence identity</term>
<term>Serine endopeptidase_putative degs stress sensor</term>
<term>Short exposure</term>
<term>Siderophore receptor</term>
<term>Signal transduction proteins</term>
<term>Similar number</term>
<term>Solar radiation</term>
<term>Solar simulator</term>
<term>Sp8h</term>
<term>Sp8h cells</term>
<term>Sp8h treatment</term>
<term>Sphingomonas alaskensis</term>
<term>Sphingopyxis</term>
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<term>Stress response protein</term>
<term>String database</term>
<term>Subunit</term>
<term>Sunlight exposure</term>
<term>Superoxide dismutase</term>
<term>Theoretical proteome</term>
<term>Total number</term>
<term>Transcription</term>
<term>Transcriptional regulation</term>
<term>Transfer flavoprotein</term>
<term>Ultraviolet radiation</term>
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<term>Abundance ratio</term>
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<term>Antitermination protein nusg</term>
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<term>Appl environ microbiol</term>
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<term>Biological replicates</term>
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<term>Blackwell publishing</term>
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<term>Cellular location</term>
<term>Cellular processes</term>
<term>Ch3cn formic acid</term>
<term>Chaperone</term>
<term>Chaperonin protein</term>
<term>Chaperonin_groes protein</term>
<term>Charge state</term>
<term>Climate change</term>
<term>Coli</term>
<term>Colour code</term>
<term>Common proteins</term>
<term>Component beta subunit</term>
<term>Computational analyses</term>
<term>Dantas caldas</term>
<term>Dark control</term>
<term>Different growth conditions</term>
<term>Different light treatments</term>
<term>Differential abundance</term>
<term>Differential protein abundance</term>
<term>Elongation factor</term>
<term>Environ</term>
<term>Environmental microbiology</term>
<term>Escherichia coli</term>
<term>Family protein</term>
<term>Fegatella</term>
<term>Formic acid</term>
<term>Functional relationships</term>
<term>Gene expression</term>
<term>Genome sequence</term>
<term>Glutamine synthetase</term>
<term>Glyoxalase</term>
<term>Growth conditions</term>
<term>Growth phase</term>
<term>Heat shock protein</term>
<term>Hierarchical cluster analysis</term>
<term>Higher abundance</term>
<term>Host factor subunit</term>
<term>Hydrogen peroxide</term>
<term>Hydrogen peroxide resistance</term>
<term>Interaction networks</term>
<term>Iron homeostasis</term>
<term>Iron storage</term>
<term>Irradiation conditions</term>
<term>Irradiation treatment</term>
<term>Irradiation treatments</term>
<term>Isobaric tags</term>
<term>Itraq</term>
<term>Itraq labelling</term>
<term>Itraq reagents</term>
<term>Joux</term>
<term>Light conditions</term>
<term>Light exposure</term>
<term>Light treatment</term>
<term>Light treatments</term>
<term>Liquid chromatography tandem mass spectrometry</term>
<term>Long exposure</term>
<term>Lower abundance</term>
<term>Manual annotation</term>
<term>Marine bacteria</term>
<term>Marine bacterium</term>
<term>Marine bacterium sphingopyxis alaskensis</term>
<term>Marine ecosystems</term>
<term>Marine environment</term>
<term>Marine oligotrophic ultramicrobacterium</term>
<term>Membrane lipoprotein</term>
<term>Metabolic pathways</term>
<term>Metabolism</term>
<term>Methanococcoides burtonii</term>
<term>Michrom bioresources</term>
<term>Microb ecol</term>
<term>Microbiol</term>
<term>Microbiology</term>
<term>Ml8h</term>
<term>Ml8h cells</term>
<term>Ml8h treatment</term>
<term>Molecular mechanisms</term>
<term>Nitrogen metabolism</term>
<term>Nucleic acids</term>
<term>Nutrient limitation</term>
<term>Ocean waters</term>
<term>Oligotrophic</term>
<term>Ompa</term>
<term>Ompa family</term>
<term>Ostrowski</term>
<term>Outer membrane protein ompa</term>
<term>Oxidative</term>
<term>Oxidative damage</term>
<term>Oxidative stress</term>
<term>Oxidative stress response</term>
<term>Pathway</term>
<term>Peptide</term>
<term>Periplasmic family protein</term>
<term>Perkin elmer</term>
<term>Pfkb family carbohydrate kinase_putative adenosine kinase</term>
<term>Photochem photobiol</term>
<term>Polymerase subunit omega</term>
<term>Positive gravy indices</term>
<term>Precursor</term>
<term>Previous studies</term>
<term>Proc natl acad</term>
<term>Protein</term>
<term>Protein abundance</term>
<term>Protein abundance changes</term>
<term>Protein dnak</term>
<term>Protein extraction</term>
<term>Protein family</term>
<term>Protein samples</term>
<term>Protein synthesis</term>
<term>Proteome</term>
<term>Proteomic</term>
<term>Proteomic analysis</term>
<term>Proteomic changes</term>
<term>Proteomic response</term>
<term>Proteomics</term>
<term>Proteomics data sets</term>
<term>Qstar pulsar</term>
<term>Quantitative differences</term>
<term>Quantitative proteomics</term>
<term>Quantitative proteomics data</term>
<term>Raftery</term>
<term>Ratio ratio</term>
<term>Ratio values</term>
<term>Reactive oxygen species</term>
<term>Receptor</term>
<term>Relative protein rate</term>
<term>Ribosomal</term>
<term>Ribosomal protein</term>
<term>Ribosomal proteins</term>
<term>Room temperature</term>
<term>Sample mixture</term>
<term>Sapc protein</term>
<term>Sequence identity</term>
<term>Serine endopeptidase_putative degs stress sensor</term>
<term>Short exposure</term>
<term>Siderophore receptor</term>
<term>Signal transduction proteins</term>
<term>Similar number</term>
<term>Solar radiation</term>
<term>Solar simulator</term>
<term>Sp8h</term>
<term>Sp8h cells</term>
<term>Sp8h treatment</term>
<term>Sphingomonas alaskensis</term>
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<term>Sphingopyxis alaskensis</term>
<term>Stationary phase</term>
<term>Stress response protein</term>
<term>String database</term>
<term>Subunit</term>
<term>Sunlight exposure</term>
<term>Superoxide dismutase</term>
<term>Theoretical proteome</term>
<term>Total number</term>
<term>Transcription</term>
<term>Transcriptional regulation</term>
<term>Transfer flavoprotein</term>
<term>Ultraviolet radiation</term>
<term>Uncharacterized</term>
<term>Uncharacterized protein</term>
<term>Viability</term>
<term>Visible light</term>
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<div type="abstract" xml:lang="en">The adaptive response of the marine bacterium Sphingopyxis alaskensis RB2256 to solar radiation (both visible and ultraviolet) was assessed by a quantitative proteomic approach using iTRAQ (isobaric tags for relative and absolute quantification). Both growth phase (mid‐log and stationary phase) and duration (80 min or 8 h) of different light treatments (combinations of visible light, UV‐A and UV‐B) were assessed relative to cultures maintained in the dark. Rates of total protein synthesis and viability were also assessed. Integrating knowledge from the physiological experiments with quantitative proteomics of the 12 conditions tested provided unique insight into the adaptation biology of UV and visible light responses of S. alaskensis. High confidence identifications were obtained for 811 proteins (27% of the genome), 119 of which displayed significant quantitative differences. Mid‐log‐phase cultures produced twice as many proteomic changes as stationary‐phase cultures, while extending the duration of irradiation exposure of stationary‐phase cultures did not increase the total number of quantitative changes. Proteins with significant quantitative differences were identified that were characteristic of growth phase and light treatment, and cellular processes, pathways and interaction networks were determined. Key factors of the solar radiation adaptive response included DNA‐binding proteins implicated in reducing DNA damage, detoxification of toxic compounds such as glyoxal and reactive oxygen species, iron sequestration to minimize oxidative stress, chaperones to control protein re/folding, alterations to nitrogen metabolism, and specific changes to transcriptional and translational processes.</div>
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<name sortKey="Raftery, M J" sort="Raftery, M J" uniqKey="Raftery M" first="M. J." last="Raftery">M. J. Raftery</name>
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<name sortKey="Cavicchioli, R" sort="Cavicchioli, R" uniqKey="Cavicchioli R" first="R." last="Cavicchioli">R. Cavicchioli</name>
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